BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Lastly, the genera Kurthia and Azotobacter were represented each by a single strain. Universidad de La Habana; The degradation potential exhibited by these new isolates warrants further studies on their possible application to decontaminate coastal environments affected by oil spills.

Biodegradation of Polycyclic Aromatic Hydrocarbons.

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Online J Biol Sci. These microorganisms can be found not only in soil and water samples, but in clinical specimens, occasionally. These compounds did not mineralize completely, accumulating instead as intermediary metabolites consisting of linear chains and aromatic compounds.

Three replicates were seeded per strain. However, and despite the higher propensity of n-alkanes for oxidation [35, 49], no differences in biodegradation percentages were detected between saturated hydrocarbons, asphaltenes and monocyclic aromatic hydrocarbons after 45 days. Hydrocarbon determinations Hydrocarbon determinations were performed at day Taxonomic scheme for the identification of marine bacteria. Biochemical characterization of the strains comprised tests for the fermentation of glucose, lactose, sucrose and mannitol, as well as for the production of indole, gas and hydrogen sulfide.

Published reports indicate that they can also degrade phenanthrene in soils [38] as well as anthracene, phenol [41] and methyl methylbromide in marine environments [40]. Total petroleum hydrocarbon TPH degradation was evaluated through SARA analysis, employing gas chromatography with an FID detector and infrared spectroscopy to analyze the aliphatic and aromatic hydrocarbon fractions, respectively. This probably explains the high number of hydrocarbon-degrading microorganisms identified through isolation protocols A and B, which totaled 33 strains.


Bioremediation potential of terrestrial fuel spills. Regardless, Pefroleo et al. Appl Environ Microbiol ;64 SPE International conference on health, safety, and environment in oil and gas exploration and production, JuneCaracas, Venezuela.

Usually found at low concentrations in non-contaminated areas, their populations bloom in chronically contaminated environments [1]. Simultaneous degradation of atrazine and phenol by Pseudomonas sp. Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil.

Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil. Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils.

In general, biodegradation is expected to be more extensive in aliphatic hydrocarbons, which are far more amenable to this process than their aromatic counterparts [35, 47, 48]. Effectiveness of the natural bacterial flora, bioestimulation and bioaumentation on the bioremediation of a hydrocarbon contaminated Antartic soil.

Monocyclic and polycyclic aromatic hydrocarbon fractions were analyzed by FTIR spectroscopy, as described in Materials and Methods. Environmental biotechnology techniques have been readily used to remediate ecological disasters caused by large oil spills, such as those caused by the collision of tanker Exxon Valdez with Bligh Reef at Prince William Sound, spilling some 50 tons of crude oil in March [6], and the sinking of Prestigewhich spilled 63 tons of crude and ended up affecting 1 km of French and Spanish coastlines in November [7].

The concentration of associated hydroxyl groups OH -gacterias -1 increased for all treatments, as did that of carboxylic groups cm Had hydrocarbon composition been determined at that point, we would have most likely found practically identical levels of asphaltenes and resins in crudes treated with the strains under examination and in their controls, significantly decreased levels of saturated hydrocarbons in the former, and only a small drop in the concentration of aromatic hydrocarbons, since the bulk of their degradation takes place after 21 days.


The latter, in turn, are more susceptible to biodegradation than resins and asphaltenes [47, 48]. Five replicates were used per sample.

BACTERIAS DEGRADADORAS DE HIDROCARBUROS by Mirelly Katherine Diaz Gamarra on Prezi

After concluding the three subculture cycles, 0. Varadero crude is higher in asphaltenes, whereas the main constituents of Pina crude are saturated hydrocarbons with chain lengths smaller than 18 carbon atoms. No alkanes with backbones shorter than 12 carbon atoms were detected in these samples. J Ind Microbiol Biot.

Molecular characterization of a toluene-degrading methanogenic consortium. No statistically significant differences were found for this parameter among the examined strains, although the increased proportion of carboxyl and hydroxyl groups demonstrates the presence of biological oxidation processes.

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Appl Environ Microbiol ;63 9: Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the dw diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3].

Each chart depicts both the profile of the strain under examination and that of the standard sample. F9S exhibited the best results regarding the degradation of asphaltenes.

Strain F10S1 degraded