IGRA STAKLENIH PERLI HESE PDF

Igra staklenih perli has ratings and reviews. Hese je te godine dobio najveće svetsko priznanje: Nobelovu nagradu za književnost. DownloadHerman hese igra staklenih perli pdf. Free Download e-Books Sony. Ericsson C – user opinions and reviews Use Reset Your PC to reinstall. Download Herman Hese – Igra staklenih perli. Description. Download Herman Hese – Igra staklenih perli Free in pdf format.

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Igra staklenih perli 1-2

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27958GE1-A MANUAL PDF

The manual is available here: asp?phone=cordless&lang=English®ion=United. Scroll down in. Get support for GE GE1 – GHz Analog Cordless Speakerphone. UPC – Free GE GE1 manuals! Problems with GE GE1?. Thank you for asking your question on JustAnswer. Please click here to view, download or print the manual. If this is not the correct manual.

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This system operates in the 2.

ge phone model 27958ge1 a manual

When the base is connected to electrical power and a telephone line, it can support a cordless handset. TVs, microwave ovens, and VCRs. To minimize or prevent such interference, the base of the cordless telephone should not be placed near or on top of a TV, microwave ovens, or VCR. If such interference continues, move the cordless telephone farther away from these appliances. Using other power supplies may damage the unit. Plug the handset charge manua, into an electrical outlet. The charge indicator on the handset charge cradle turns on to indicate the handset battery is charging Allow the handset to charge for 12 hours prior to first use.

Feed the telephone line cord and power supply cord through the grooves on the 27958be1-a to allow the unit to rest flat against the wall. Use the touch-tone pad to enter your new 3-digit security code. For example, press the 6, 2, and 8 number keys for security code The default security code is This setting may only be made with the base.

Most telephone systems use tone dialing, however, some may still use pulse dialing. Depending on your telephone system, set the dialing mode as follows: This setting may be made with the base or the cordless handset.

Lift the handset and wait for a dial tone. Dial the desired number.

Dial the number, then lift the handset. When finish, put the handset back on the cradle to hang up. The base ringer volume is set through the programming menu.

Refer to Telephone Set Up for more information. If you get a busy signal, and want to keep dialing the number, press the redial button to quickly redial 27598ge1-a number again. Press the HOLD button on the base to put a call on hold. The hold indicator light turns on. To take a 27958e1-a off hold, press the HOLD button again, and the hold indicator light turns off.

GE GE1 – GHz Analog Cordless Speakerphone Manual

Using the temporary tone allows you to temporarily make your phone touch tone compatible. Dial the telephone number and wait for the line to connect. The microphone might pick up these sounds. Speak into the handset. Base – Speakerphone rings. Use the touch-tone pad to enter the area code followed by the telephone number up to manuxl digits, including pauses and press the memory button again to save your selection.

Follow the steps under Storing a Name and Number in Memory except replace the stored name or number with the new information. Make sure the phone is OFF not in talk mode. If the number is corrupted, such as a number in a name or an alphabetical character in a number, an error tone comes from the handset and the number won’t dial.

The CID information appears in the display after you hear the tone. Begin speaking after you hear the beep. Release the button after you finish your announcement. If you choose not to record an outgoing announcement, the default announcement plays. Release the MEMO button after you are finished. When the answering system picks up: Listen as the caller leaves a message. You can bypass the outgoing announcement by pressing any numbered key on the touch-tone pad while the announcement is playing.

Then, you can enter your security code to access the answering system.

GE 27958B User Manual

Like other batteries of this type, toxic materials could be released which can cause injury. To reduce the risk manuap fire or personal injury, use only the battery listed in the instruction book. Ensure the base power cord is connected to a working outlet.

Ensure the telephone line cord is connected to the base and the wall jack. The battery may need to be charged. Manul If trouble is experienced with this equipment, for repair or warranty information, please contact customer service at If the equipment is causing harm to the telephone network, the telephone company may request that you disconnect the equipment until the problem is resolved.

This product may be serviced only by the manufacturer or its authorized service agents. For how long after your purchase: The warranty period for 27958ye1-a units begins with the first rental or 45 days from date of shipment to the rental firm, whichever comes first. What we will do: Este sistema opera a un rango de frecuencia de 2. Placa para montaje no incluida.

Cuando haya terminado, ponga nuevamente el auricular en mnaual sitio para colgar. Siga las siguientes pautas cuando utilice el altavoz: El indicador de mensajes nuevos parpadea cuando usted tiene un mensaje nuevo.

Coloque el auricular sobre el cargador para que se cargue. Page of manusl Go. Answering System with Cordless Handset. We bring good things to life. Table of Contents Add to my manuals Add. Cordless Telephone GE Manual 58 pages.

ge1-a User Manual – GE GE1 2 4 GHz Analog Cordless Speakerphone

2758ge1-a Telephone Set Up 4. Rings To Answer 1. Local Area Code 6. Base Unit The base ringer volume is set through the programming menu. Hold If you get a busy signal, and want to keep dialing the number, press the redial button to quickly redial the number again. Olume when you call your bank you may need to enter your account number. Chain Dialing From Memory -Or- 27958te1-a.

Ull When the answering system picks up: Service ERVICE If trouble is experienced with this equipment, for repair or warranty information, please contact customer service at Timbres Para Contestar 2. Ecibir Una Siga las siguientes pautas cuando utilice el altavoz: Para Introducir Unap 4.

Indicador Del Sistemac 2. This manual also for: Comments to this Manuals Your Name. Enter text from picture: By pressing ‘print’ button you will print only current page. To 27958ve1-a the manual completely, please, download it. Don’t show me this message again. Got it, continue to print. Don’t have an account?

DAV-HDX265 MANUAL PDF

Audio manuals and audio service pdf instructions. Find the user manual you Sony DAV-HDX Home Theater System User Manual. Page 1. Page 2. Page 3 . Download Manual for Model DAV-HDX SONY DVD HOME THEATER PartsDirect has parts, manuals & part diagrams for all types of repair. Dvd home theatre system • Read online or download PDF • Sony DAV-HDX User Manual.

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Using the online preview, you can quickly view the contents and go to the page where you will find the solution to your problem with Sony DAV-HDX If looking through the Sony DAV-HDX user manual directly on this website is not convenient for you, there are two possible solutions:. Many people prefer to read the documents not on the screen, but in the printed version.

The option dav-hdc265 print the manual has also been provided, and you can use it by clicking the link above – Print the manual. If you want to quickly view the content of pages found on the following pages of the manual, you can use them.

Sony DAV-HDX265 Operating Instructions Manual

majual These limits are designed to shock, do not expose this apparatus to provide reasonable protection against harmful rain or moisture. When a cart is used, use caution Theatre System.

Inserting batteries into the remote You can control the system using the supplied mankal. Insert two R6 size AA batteries by matching the 3 and ends on the batteries to the markings inside the compartment.

Sony DAV-HDX265 Home Theater System User Manual

To use the remote, point it at the remote sensor on the unit. For other TV connections, see page For other component connection, see manuzl To accept progressive signals, see page Refer to the connection diagram below, and read the additional information from 1 to 4. Positioning the recommended that you change the settings. Positioning the speakers For the best possible surround sound, all the speakers other than the subwoofer s.

The Setup Display for. Set up the calibration mic at the ear level Auto Calibration st.

See the illustrations 4. For your convenience If looking through the Sony DAV-HDX user manual directly on this website is not convenient for you, there are two possible solutions: Full Screen Viewing – to easily view the user manual without downloading it to your computeryou can use full-screen viewing mode.

However, if you do adv-hdx265 want to take up too much of your disk space, you can always download it in the future from ManualsBase.

Summary of the content on the page No. This equip Summary of the content on the page No. Refer to the connection diagram below, and read the additional information from 1 to 4 Summary of the content on the page No.

Sony Dav-hdx Hdx Hdxw Home Theater System Owners Manual | eBay

Positioning the speakers For the best possible surround sound, all the speakers other than the subwoofer s Summary of the content on the page No. The Setup Display for Summary of the content on the page No. Set up the calibration mic at the ear level Auto Calibration st Summary of the content on the page No.

LE POLYGONE DE WILLIS PDF

Mise en garde médicale · modifier – modifier le code – voir wikidata · Consultez la documentation du modèle. La maladie de moyamoya (appelée aussi moyamoya, maladie de Nishimoto, maladie de Nishimoto-Takeuchi-Kudo ou maladie obstructive du polygone de Willis). Le cercle cérébral artériel (circulus artériel cérébral), également connu sous le cercle de Willis (GC), boucle de Willisor Willis polygone) a été décrite par. Download scientific diagram | 2-Polygone de Willis. (1) Artère communicante antérieure. (2) Artère carotide interne. (3) Artère cérébrale postérieure. (4) Artère .

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The circle of Willis also called Willis’ circleloop of Williscerebral arterial circleand Willis polygon is a circulatory anastomosis that supplies blood to the brain and surrounding structures. It is named after Thomas Willis —an English physician. The circle of Willis is a part of the cerebral circulation and is composed of the following arteries: The middle cerebral willkssupplying the brain, are not considered part of the circle.

The left and right internal carotid arteries arise from the left and right common carotid arteries. The posterior communicating artery is given off as a branch of the internal carotid artery just before it divides into its terminal branches – the anterior and middle cerebral arteries. The anterior cerebral artery forms polyogne anterolateral portion of the circle of Willis, while the middle cerebral artery does not contribute to the circle.

The right po,ygone left posterior cerebral arteries arise sillis the basilar arterywhich is formed by the left and right vertebral arteries. The vertebral arteries arise from the subclavian arteries. The anterior communicating artery connects the two anterior cerebral arteries and could be said to arise from either the left or right side. All arteries involved give off cortical and central branches.

Polygone de Willis

The central branches supply the interior of the circle of Willis, more specifically, the Interpeduncular fossa. The cortical branches are named for the area they supply.

Since they do not directly affect the circle of Willis, they are not dealt with here. Considerable anatomic variation exists in the circle of Willis. Based on a study of brains, the classic anatomy of the circle is only seen in In another variation the anterior communicating artery is a large vessel, such that a single internal carotid supplies both anterior cerebral arteries; this is known as an azygos anterior cerebral artery.

The arrangement of the brain’s arteries into the circle of Willis creates redundancy analogous to engineered redundancy for collateral circulation in the cerebral circulation. If one part of the circle becomes blocked or narrowed stenosed or one of the arteries supplying the circle is blocked or narrowed, blood flow from the other blood vessels can often preserve the cerebral perfusion well enough to avoid the symptoms of ischemia.

File:Polygone de Willis.png

The redundancies that the circle of Willis dee can also lead to reduced cerebral perfusion. Subclavian steal syndrome results from a proximal stenosis narrowing of the subclavian arteryan artery supplied by the aortawhich is also the same blood vessel that eventually feeds the circle of Willis via the vertebral artery.

From Wikipedia, the free encyclopedia. Circle of Willis Schematic representation of the circle of Willisarteries of the brain and brain polyone.

Blood flows up to the brain through the vertebral arteries and through the internal carotid arteries. The arteries of the base of the brain. Basilar artery labeled below center.

The temporal pole of the cerebrum and the cerebellar hemisphere have been removed on the right side. Inferior aspect viewed from below. Thomas Willis’ Famous Eponym: Turkish Journal of Medical Sciences.

Retrieved 16 July Augustine; David Fitzpatrick; William C. Archived from the original on Accessed on November 6, Correlation with cerebral collaterals in internal carotid artery occlusive disease”. A Journal of Cerebral Circulation. This article polygohe anatomical terminology; for an overview, see anatomical terminology. Arteries of the head and neck. Circle of Willis ACA anterior communicatingRecurrent artery of HeubnerOrbitofrontal polygnoe MCA anterolateral centralPrefrontal arterySuperior terminal branchInferior terminal branchAnterior temporal branch posterior communicating anterior choroidal.

Retrieved from ” https: Commons category link is on Wikidata Wikipedia articles with TA98 identifiers. Views Read Edit View history.

In other projects Wikimedia Commons. This page was last edited on 27 Novemberat By using this site, you agree to the Terms of Use and Privacy Policy.

Schematic representation of the circle of Willisarteries of the brain and brain stem. Anatomical terminology [ edit on Wikidata ]. Wikimedia Commons has media related to Circle of Willis.

AIRTHREAD CONNECTIONS PDF

one of the largest cable operators in the U.S., prepared to acquire the AirThread. Connections (ATC), a large regional cellular provider. Although this acquisition. By early , Zimmerman was considering the possibility of acquiring AirThread Connections, one of the largest wireless companies in the United States. This case can be used as a capstone valuation exercise for first-year MBA students in an introductory finance course. A senior associate in the business.

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We use cookies to give you the best experience possible. Briefly explain your answer. First, American Cable Communication ACC and AirThread could help each other compete in the industry that was moving more and more bundled service offerings.

Second, the acquisition could help both companies expand into the business market. The reasons above make us believe that the synergy is positive and the acquisition is a good idea. Based on the projected cash flow information provided in the case, what is the stand- alone value of AirThread? Show the cash flow forecasts, discount rate, and your valuation model.

Valuation of AirThread Connections

For example, Accounts Receivable First, when we calculate the operating value, we use the DCF model. We pick the risk-free rate from historical annual returns investments on T-bonds from to and use the geometric airthreadd, which is 5. We assume the equity as the average equity of the industry, which is 0. Finally, we get the WACC, which is 9.

Accounts receivable is based on total revenue. Rev is based on equipment revenue. Exhibit 2 According to the casebook, the reinvestment rate is defined as capital expenditures plus investments in working capital minus depreciation divided by net operating profit after taxes, and the ROC return on capital is defined as connectikns operating profit after taxes divided by the book value of equity plus debt.

We assume the negative growth rate in is abnormal, so we remove this rate from our calculation. Next, we use the market multiple approach to calculate the non-operating value part. And we use the equity in earnings of affiliates of AirThread to multiple Given the projected synergy and financing method, what is the value of AirThread as a merger target?

What methodology should be used to value AirThread, given the characteristics of the expected cash flows after the merger? The debt payment schedule is presented in Ex 6.

Remember that different valuation models are not mutually exclusive, you can use different model for different forecasting periods. First, we calculate the operating value during and using APV. The cash flows of airtheead five years combine the stand-alone cash flows and the arthread cash flows. First of all, we calculate the NPV. The potential synergies come from system operating cost saving as well as the increase in revenue and gross profit.

We assume that after the acquisition, the constant growth rate will increase to 2. Besides the three advantages we mentioned before, after calculation, we find that the value of AirThread aifthread a merger target is higher than the stand-alone value of AirThread.

Featured case – Valuation of AirThread Connections | The Case Centre, for educators

We conduct the sensitivity analysis to find the effect on the value of AirThread by changing two inputs. We choose to change the WACC and growth rate to achieve this because we think those variables are most important.

Valuation of AirThread Connections. Accessed December 31, We will write a custom sample essay on Valuation of AirThread Connections specifically for you. Leave your email and we will send you an example after 24 hours If you contact us after hours, we’ll get back to connctions in 24 hours or less. Hi there, would you like to get such a paper? How about receiving a customized one? Click to learn more https: Valuation of AirThread Connections Essay.

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Valuation of AirThread Connections Essay

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CLIPPER AND CLAMPER CIRCUITS PDF

Recall Lecture 6 • Rectification – transforming AC signal into a signal with one polarity – Half wave rectifier • Full Wave Rectifier – Center tapped – Bridge. Section B8: Clippers And Clampers. We’ve been talking Clippers. Clipping circuits (also known as limiters, amplitude selectors, or slicers), are used to remove. Differentiates between a cliiper circuit and a clamper circuit in both parallel and series connections.

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A c,amper is an electronic circuit that changes the DC level of a signal to the desired level without changing the shape of the applied signal. In other words, the clamper circuit moves the whole signal up or down to set either the positive peak or negative peak of the signal at the desired level. The dc component is simply added to the input signal or subtracted from the input signal.

A clamper circuit adds the positive dc component to the input claamper to push it to the positive side. Similarly, a clamper circuit adds the negative dc component to the input signal to push it to the negative side. If the circuit pushes the signal upwards then the circuit is said to be a positive clamper. When the signal is pushed upwards, the negative peak of the signal meets the zero level.

On the other hand, if the circuit pushes the signal downwards then the circuit is said to be a negative clamper. When the signal is pushed downwards, the positive peak of the signal meets the zero level. The construction of the clamper circuit is almost similar to the clipper circuit. The only difference is the clamper circuit contains an extra element called capacitor.

A capacitor is used to provide a dc offset dc level from the stored charge. A typical clamper is made up of a capacitor, diodeand resistor. Some clampers contain an extra element called DC battery. The resistors and capacitors are used in the clamper circuit to maintain an altered Clampdr level at the clamper output.

The clamper is also referred to as a DC restorer, clamped capacitors, or AC signal level shifter.

Clipper and Clamper Circuit

Clamper circuits are of three types: The positive clamper is made up of a voltage source V icapacitor C, diode D, and load resistor Xircuits L. In the below circuit diagram, the diode is connected in parallel with the output load.

So the positive clamper passes the input signal to the output load when the diode is reverse biased and blocks the input signal when the diode is forward biased. During negative half cycle: During the negative half cycle of the input AC signal, the diode is forward biased and hence no signal appears at the output.

In forward biased condition, the diode allows electric current through it. This current will flows to the capacitor and charges it to the peak value of input voltage V m. The capacitor charged in inverse polarity positive with the input voltage. As circukts current or voltage decreases after attaining its maximum value -V mthe capacitor holds the charge until the diode remains forward biased. During positive half cycle: During the positive half cycle of the input AC signal, the diode is reverse biased and hence the signal appears at the output.

In reverse biased condition, the diode does not allow electric current through it. So the input current directly flows towards the output. When the positive half cycle begins, the diode is in the non-conducting state and the charge stored in the capacitor is discharged released. As a result, the signal shifted upwards.

Clipper (electronics)

The peak to peak amplitude of the input signal is 2V m snd, similarly the peak to peak amplitude of the output signal is also 2V m. Therefore, the total swing of the output is same as the total swing of the input.

The basic difference between the clipper and clamper is that the clipper removes the unwanted portion of the input signal whereas the cliipper moves the input signal upwards or downwards. During the positive half cycle of the input AC signal, the diode is forward biased and hence no signal appears at the output.

This current will flows to the capacitor and charges it to the peak value of input voltage in inverse polarity -V m.

Clamper circuits – Positive clamper, Negative clamper and Biased clamper

As input current or voltage decreases after attaining its maximum value V mthe capacitor cllamper the charge until the diode remains forward biased. During the negative half cycle of the input AC signal, the diode is reverse biased and hence the signal appears at the output.

When the negative half cycle begins, the diode is in the non-conducting state and the charge stored clamepr the capacitor is discharged released. As a result, the signal shifted downwards. Sometimes an additional shift of DC level is needed. In such cases, biased clampers are used. The working principle of the biased clampers is almost similar to the unbiased clampers. The only difference is an extra element called DC battery is introduced in biased clampers.

If positive biasing is applied to the clamper then it is said to be a positive clamper with positive bias. The positive clamper with positive bias is made up of an AC voltage source, capacitor, diode, resistor, and dc battery. During the positive half cycle, the battery voltage forward biases the diode when the input supply voltage is less than the clippper voltage. This current or voltage will flows to the capacitor and charges it. When the input supply voltage becomes greater than the battery voltage then the diode stops allowing electric current through it because the diode becomes reverse biased.

During the negative half cycle, the diode is forward biased by both input supply voltage and battery voltage. So the diode allows electric current. This current will flows to the capacitor and charges it. During the negative half cycle, the battery voltage reverse biases the diode when the input supply voltage is less than the battery voltage.

As a result, the signal appears at the output. When the input supply voltage becomes greater than the battery voltage, the diode is forward circhits by the input supply voltage and hence allows electric current through it.

During the positive half cycle, the diode is reverse biased by both clampeer supply voltage and the battery voltage. The signal appeared at the output is equal to the sum of the input voltage and capacitor voltage. During the positive half cycle, the battery voltage reverse biases the diode when the input supply voltage is cla,per than the battery voltage. During the negative half cycle, the diode is reverse biased by both input supply voltage and battery voltage.

During the positive half cycle, the diode is forward biased by both input supply voltage and battery voltage. As a result, current flows through the capacitor and charges it.

During the negative half cycle, the battery voltage forward biases the diode when the input supply voltage is less than the battery voltage. When the input supply voltage becomes greater than the battery voltage, the diode is reverse biased by the input supply voltage and hence signal appears at the output. Clamper definition A clamper is an crcuits circuit that changes the DC level of a signal to the desired level without changing the shape of the applied signal.

Rectifier Rectifier – What is rectifier Half wave rectifier Half wave rectifier with filter Full wave rectifier Full wave rectifier with filter Bridge rectifier Bridge rectifier with filter Clipper circuits Clamper circuits Voltage multiplier.

Types of clampers Clamper circuits are of three types: Positive clampers Negative clampers Biased clampers Positive clamper The positive clamper is made up of a voltage source V icapacitor C, diode D, and load resistor R L. Negative clamper During positive half cycle: Biased clampers Sometimes an additional shift of DC level is clippee.

Positive clamper with positive bias If positive biasing is applied to the clamper then it an said to be a positive clamper with positive bias.

Positive clamper with negative bias During negative half cycle: Negative clamper with positive bias During positive half clipler Negative clamper with negative bias During positive half cycle:

ANTICUERPOS ANTIDIGOXINA PDF

dewiki Digitalis-Antidot; enwiki Digoxin immune fab; eswiki Anticuerpos antidigoxina; plwiki Digitalis-Antidot; shwiki Digoksin imun Fab; srwiki Digoksin imun Fab. In life-threatening situations, antidigoxin antibodies must be used. caciones de los anticuerpos antidigoxina en la intoxicación digitálica. Revisio ́n sistema ́tica sobre la efectividad e indicaciones de los anticuerpos antidigoxina en la intoxicacio ́n digita ́lica. [Systematic review of the effectiveness .

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In general, digoxin is a drug used to treat cardiac disorders. It is a digitalis, i. It has a positive inotropic effect, i. The resulting increase of calcium into the cell causes an increase in amtidigoxina excitability of cardiomyocytes, causing an increase in cardiac contractility. Digoxin has a narrow therapeutic index, with therapeutic level very close to the toxic level.

To combat its toxic effect may be. In a more specific form, the cardiac glycoside digoxin is the most used in the treatment of congestive heart failure and atrial fibrillation Figure 1. Research and experience with digoxin began over years Withering, and its application in patients with heart failure have been reported only in the 20th century, defining its ability to increase the contractility of the heart. This membrane protein is responsible for sodium transport to the extracellular milieu and be inhibited, generates an increase abticuerpos intracellular sodium.

This stimulates increased by exchanging the sodium-calcium pump, increasing the concentration of intracellular calcium by contractile proteins used. It is an inexpensive anticuerpod, it is important in developing countries where patients do not have access to sophisticated therapies Gheorghiade et al.

The concentration of digoxin in the serum depends not only on the dose administered, but is also related to interactions with other drugs and the patient’s condition Antman, Smith, Digoxin is not antigenic because it is a small molecule InSmith et al.

They documented high affinity and specificity of selected antibodies to haptens populations of cardiac glycosides. Fab fragments Fragment antigen binding antibodies specific for digoxin obtained in sheep demonstrated ability to revert digoxin poisoning Smith et al. A study in dogs showed that intravenous administration of purified Fab portion of the anti -digoxina sheep polyclonal antibodies can quickly reverse the cardiotoxicity, connecting to the free digoxin in plasma and causing a redistribution of the drug from tissues back to blood circulation.

Fab fragments are relatively fast excreted in urine, so Fab high affinity, which retain binding with the drug, can provide antidigoxinz route of drug elimination, as well as means to neutralize it Butler et al. Therapies with potassium and beta-blockers have been used as agents to combat alternative to digitalis intoxication, but the administration of Fab fragments of antibodies is the main therapy to reverse the intoxication ANTMA et al.

The Fab fragment of polyclonal anti-digoxin antibody raised in sheep is being manufactured only by a company Protherics under the name digifab and received FDA approval in The use of Fab fragments in cases of poisoning by digoxin is safe and effective, but the neutralization with Fab fragments is an expensive therapy, is used only when other treatment options seem to fail.

Its high cost also limits its availability to all hospitals or countries. Namely, the antibodies are glycoproteins composed of two light chains LC, light chain identical approximately 24 kDa each and two heavy HC, heavy chain identical kDa each.

A light chain is covalently linked to a heavy chain by a disulfide bond, while the two heavy chains are linked together by disulfide bridges. The carboxy terminal regions of the heavy chains perform effector functions. The light and heavy chains have an amino-terminal variable region VL and VH va iable light, heavy variable which is involved in recognition of antigens. Among these regions are present more conserved framework calls sequences or regions framework: IgG, with molecular weight kDa, is the most abundant in mammalian serum.

The IgGl is most commonly found in human serum Abbas Lichtman, and has well-defined structure with two disulfide bonds linking the heavy chains in the hinge region between CHI and CH2.

InKohler and Milstein monoclonal antibodies developed production technology Acmoswhich gave them the Nobel Prize in Physiology and Medicine in Many murine monoclonal antibodies have been produced for treatment or diagnosis of human diseases.

However, its use is limited by having short half-life in serum and due to its high immunogenicity, and may cause a reaction known as human anti-mouse antibody HAMA. Antibody fragments are more advantageous for certain clinical applications that do not rely on whole antibody. Maintain the antigen-binding sites, are less immunogenic than q and whole IgG, the best diffuse interstitial space, is easily eliminated through glomerular filtration and stocks are more stable than IgG Flanagan, Jones, May be obtained by digestion of immunoglobulin with pepsin enzyme that produces two Fab fragments linked by dissulteto bridge, F ab ‘ 2 divalent weight of approximately kDa and fragmented Fc portion or by the enzyme papain to produce two Fab fragments monovalent, having molecular weight of 50 kDa and the entire Fc portion Figure 2.

The anti-digoxin Fab fragments commercially available were obtained by using IgG digestion the enzyme papain. Antibody fragments may also be obtained by expression in Escherichia coli, offering the advantage of obtaining in large quantities at low cost.

Digoxin Immune Fab (Ovine) – Wikidata

Recombinant DNA technology also offers the opportunity to introduce mutations in the gene sequences that can be advantageous. Phage display technology includes a method widely used in the generation of antibody fragments. It emerged in when George Smith demonstrated that the correlation between genotype and phenotype could be established on bacteriophage phage filamentous Figure 3. Exogenous peptides exposed on the surface of phage could be selected by affinity for the specific antibody, allowing them to be enriched compared to the original peptide, usually by a process called panning.

InHuse et al reported the generation of a Fab fragment library in lambda phage from the random combination of heavy and light chains of the murine antibody as a method that could replace hybridoma technology. The technology allows selection of a phage clone displaying high affinity and specificity antibody fragments for a particular antigen within a phage library constructed from rearrangement of the variable domains Clackson et al.

Construction of the antibody phage display technology began with the construction of a library of immunoglobulins which can be generated from immunized animals, immunized or non-synthetic library STRACHAN et al. The filamentous phage of the Ff class fl, fd and M13 have 11 genes I – XI and of these five encoding capsid proteins that may be fused to the recombinant proteins of interest with greater or lesser degrees of success, with pIII the more used for phage display.

Proteins may also be exposed to phage small particles called phagemids, which are plasmids that carry the recombinant capsid protein gene and contains origin of replication of phage M13 Barbas et al. The capsid fusion protein is exposed on the phagemid particles containing the helper phage genomes. These particles containing the two genomes may be selected by selection markers Barbas et al. The helper phage has a defective origin of replication and its genome is packed inefficiently compared to phagemid Barbas et al.

After selection and characterization of monoclonal antibody, the phage display technology allows the manipulation of the antibody genes by the generation of mutants. Phage display is a handy technology in molecular biology laboratories.

It is not, however, commonplace technology and many details need to be worked to arrive at a good result. Due to the advantages of the phage display technology and the importance of anti -digoxina antibodies in cases of poisoning and commercial production is foreign and expensive, applicants believe in the need for the development and production of this drug in Brazil.

A brief survey to databases of patents on the subject “ant i monoclonal -digoxina” resulted in some documents, such as the North American US – The General Hospital Co. DNA genome ; source: It is engineered to digoxin monoclonal human antibody chain used to detect the application of digoxin concentration in patient serum and detecting the resulting toxic effect antagonist against digoxin patient poisoned by digoxin.

The present patent of invention relates, in a broader view, a production method and obtaining the antibody anti-digoxin Fab clones using phage display technology and characterization of their binding to antigen. More specifically, the invention has the following objectives:. Were znticuerpos, materials and methods angidigoxina specially for obtaining clones of anti-digoxin antibody Fab fragment using the technology antidugoxina ‘phage display’. Unlike commercial products imported.

Thus, the invention is dedicated, in particular, the development of therapeutic product for antidiogxina with specific anitcuerpos and more precise dose to detoxification of patients receiving digoxin. The complement the present description in order to obtain a better understanding of the characteristics of the present invention and according to a preferred practical embodiment thereof, accompanying description, in the annex, a set of drawings where, exemplified way, although not limiting, it represented its operation:.

Figure 2 – Structure of the IgG molecule and fragments thereof which retains antigen-binding sites; immunoglobulin fragments presented: Figure 3 – Schematic representation of the filamentous bacteriophage Ff class and its capsid proteins; presented the capsid proteins can be used for expression of recombinant proteins in the form of fusion protein by phage display technology.

Figure 5 – Schematic representation of the main stages of the display and panning phage; the Fab library was transformed into E. Phages not bound were discarded by washing and bound phages were eluted which i and used to re-infect E. The secondary antibody used was anti-mouse-IgG conjugated with peroxidase. Figure 9 – Xnticuerpos of amplification of the cDNA using oligonucleotides hybridoma -digoxina anti murine immunoglobulin, where the amplifications were made by PCR and analyzed by electrophoresis on 1.

B Products of amplification of genes using oligonucleotide LC Mok 3 ‘and the six oligonucleotides 5’. Figure 15 – a symbolic representation of the amino acid sequences deduced from clones of CL atnicuerpos, 2, 9 and 10, after phage display selected; amino acid residues have been replaced by the symbols and CDR2 regions FR1 to highlight the different sequences.

The other regions have identical sequences to each other. Figure 16 – electrophoretic profile of clones 1, 2. Figure 17 – Western blotting of crude extracts containing Fab fragments of anti -digoxina clones 1, 2, 9 and.

After electrophoresis, the gel was transferred to PVDF membrane. The membrane was incubated with anti-mouse IgG atnicuerpos specific for F ab ‘ 2 conjugated to peroxidase and detected with the ECL system. After electrophoresis the gel was stained with silver. Four gels with the same samples used in A were transferred to PVDF membranes and each membrane anticuerpox incubated with crude extracts of E.

Then the membranes were incubated with anti-mouse IgG antibody specific for F ab ‘ 2 conjugated to peroxidase and developed with ECL system.

Figure 19 – Profile of the binding of anti-digoxin Fab expressed anticuerpoe E. Fab present in crude extracts of E.

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The secondary antibody used was anti-mouse IgG specific for F ab ‘ 2 conjugated to peroxidase; and. Crude extracts of clones containing Fab expressed in E.

The relative response was measured 5 seconds, after the completion of the application of the analyte, at the point indicated by the red arrow clone Step 2 – Obtaining monoclonal antibody anti -digoxina; Step 2. Step 3 – Construction of Fab library in phagemid pComb3X from anti -digoxina hybridomas. Step 5 – Expression of soluble Fab fragments; Step 5.

Step 6 – Characterization of crude extracts containing Fab fragments from the clones obtained by phage display. To anhidigoxina define each step and their respective phase, is described below the method in its comprehensive feature, which can be accompanied by the already related illustrations:. The protocol was based on the technique described by Erlanger and Beiser After 30 minutes of interaction, 60 ul of 1 M glycerol was added to inactivate excess periodate and the solution was stirred for 5 minutes.

The periodate oxidized digoxin the solution was added to the BSA solution slowly and dropwise, keeping the pH between 9. The solution was continued stirring for 1 hour at room temperature, keeping the pH stable for 30 minutes in the range 9. Was added 6 mg of NaBH 4 dissolved in 2 mL deionized water prepared immediately. Antidigpxina estimate the number of molecules of digoxin conjugated to BSA was used as a reference absorbance at a wavelength of nm from a known concentration of digoxin solution, in duplicate.

The reference and sample Dig-BSA were completed with water up to 2 mL, then 10 mL of H2S04 were added and the tubes were kept on standing at room temperature for 4 hours. Read the absorbance was taken and calculated the number of moles of digoxin present in Dig-BSA conjugate. The estimated number of molecules present in the digoxin conjugate was obtained from antidgoxina ratio between the number of Dig-BSA digoxin mole and number of moles BSA added to obtain the conjugate.

The viable cell count was done with the aid of the dye Trypan blue exclusion and a Neubauer chamber. After loading, passed by the column equilibration buffer to remove any non-specific binding and rebalance the spine.

CA IPCC LAW BOOK MUNISH BHANDARI PDF

Bestword Handbook Law,Ethics, & Communications Munish Bhandari Bestword Bestword A Handbook on Law, Ethics and Communication for CA IPCC (Old The Book includes a ‘Trend’ showing distribution of marks in Past 10 CA (Final). Book. A Handbook On Corporate And Other Laws For Ca Intermediate (Ipcc) New Syllabus By Munish Bhandari Applicable For May Exams. Author. Author: Munish Bhandari; Language: English; Publisher: Bestword for CA- IPCC: A Self Study Textbook with Quick Revision Book (Group.

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DIN 2999-1 PDF

DIN BSPT (British Standard Tapered Pipe). Specifications of International Thread Standards. ○. Whitworth Tapered Pipe Thread. Whitworth Pipe Thread. Buy DIN PIPE THREADS FOR TUBES AND FITTINGS – PARALLEL INTERNAL THREAD AND TAPER EXTERNAL THREAD – TREAD. Buy DIN E: PIPE THREADS FOR TUBES AND FITTINGS – PARALLEL INTERNAL THREAD AND TAPER EXTERNAL THREAD.

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This is an incomplete list of DIN standards. From Wikipedia, the free encyclopedia. This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources.

BSPT Whitworth Tapered Pipe Thread DIN EN and DIN EN

Unsourced material may be challenged and removed. May Learn how and when to remove this template message. Municipal Services Standards Committee. Retrieved from ” https: Articles needing additional references from May All articles needing additional references Use dmy dates from March Articles containing German-language text. Views Read Edit View history. In other projects Wikimedia Commons. This page was last edited on 11 Decemberat By using this site, you agree to the Terms of Use and Privacy Policy.

ISO general purpose metric screw threads – Part 1: ISO general purpose metric screw threads – Part 2: ISO general purpose metric screw threads – Part 3: ISO general purpose metric screw threads – Part 4: ISO general purpose metric screw threads – Part 5: ISO general purpose metric screw threads – Part 6: ISO general purpose metric screw threads – Part 7: ISO general purpose metric screw threads – Part 8: ISO general purpose metric screw threads – Part 9: ISO general purpose metric screw threads – Part Combination of tolerance classes for formed internal screw threads.

External screw threads for transition fits former: Multi-start thread tolerances and deviation of profile. ISO metric trapezoidal screw threads; gauging of external and internal threads; gauge dimensions and design features.

ENEN Product grade A washers – with a hardness up to HV designed for use with hexagon head bolts and nuts. Fasteners – Part 2: Fasteners – Part 3: Technical delivery conditions; Property classes for carbon steel and alloy steel bolts and screws; Conversion of property classes.

Fasteners — Part 5: Technical delivery conditions; Acceptance inspection; modified version of ISOedition. Fasteners – Part 6: Technical delivery conditions; Designs and accuracy of measurement for product grade F.

Fasteners – Part Technical delivery conditions with additions to ISO ; Corrosion-resistant stainless steel fasteners. Technical delivery conditions; Property classes for nuts with fine thread ISO classes. Technical delivery conditions; Property classes for nuts Hardness classes.

Technical delivery conditions; Torsion testing of M1 to M10 bolts and screws. Technical specifications; Steel screws, bolts and studs with adhesive coating. Technical specifications; Steel screws, bolts and studs with locking coating. Technical specifications; Metric thread rolling screws of property class Engineering drawing practice; dimensioning; tolerancing of linear and angular dimensions modified version of ISO Gas cylinder valves for cylinder test pressures up to bar – Part 1: Valve inlet and outlet connections.

Standardization – Part 2: Presentation of standards; examples for presentation of figures, tables and part lists. Standardization – Part Hexagon slotted nuts and castle nuts with metric coarse and fine pitch thread – Product grades A and B.

Hexagon thin slotted nuts and castle nuts with metric coarse and fine pitch thread – Product grades A and B.

Hot rolled I-sections – Part 1: Narrow flange I-sections, I-serie – Dimensions, masses, sectional properties. Hot rolled I-beams – Part 2: Wide flange I-beams, IPB-serie; dimensions, masses, sectional properties. Hot rolled I-beams; wide flange I-beams, light pattern, IPBl-serie; dimensions, masses, fin properties. Hot rolled I-beams; wide flange I-beams heavy pattern, IPBv-serie; dimensions, masses, sectional properties.

Letter symbols for physical quantities – Part 9: Symbols for use in the field of static converters using semiconductor devices. Symbols for equivalent circuits of piezoelectric crystals.

Mechanical vibrations, oscillations and vibration systems – Part 2: Linear vibration systems with single degree of freedom. Mechanical vibrations, oscillations and vibration systems – Part 3: Linear time-invariant vibration systems with a finite number of degrees of freedom.

Basic Concepts in Metrology — Part 3: Basic Concepts in Metrology — Part 4: Treatment of Uncertainties in the Evaluation of Measurements.

List of DIN standards

Vocabulary for value management, value analysis and functional analysis – Part 1: Value analysis and functional analysis. Bibliographic references to documents – Part 1: Full References to Printed and Published Documents. DIN Part 1. DIN Part 3. General provisions relating to the award of construction contracts. General conditions of contract relating to the execution of construction work. Alphanumeric office machines – Alphanumeric keyboards, keyboard arrangement for manually operated typewriters.

Alphanumeric office machines; alphanumeric keyboards, keyboard arrangement for electro-mechanical operated typewriters. Keyboards for data and text input – Part 1: Pipe threads for tubes and fittings; parallel internal thread and taper external thread; tread dimensions.

Pipe threads for tubes and fittings; sizes for taper screw limit plug gauges for parallel internal thread. Fire behaviour of building materials and elements – Part 1: Classification dim building materials – Requirements and testing.

Building Components; Definitions, Requirements and Tests.

Fire behaviour of building materials and elements – Part 4: Overview and design of classified building materials, elements and components. Ventilation Ducts; Definitions, Requirements and Tests. Fire behaviour of building materials and elements – Part 7: Roofing – Concepts, requirements and testing. Fire behaviour of building materials and elements – Part 9: Fire behaviour of building materials and building components – Part Fire behaviour of building materials and elements – Part Fire resistance of electric cable systems required to maintain circuit integrity – Requirements and testing.

Fire resistant glazing; concepts, requirements and testing. Determination of the burning behaviour of floor covering systems using a radiant heat source. Fire behaviour of building materials and components – Part Application standard for DIN based on the design of partial safety factors.

Thermal protection and energy economy in buildings – Part 2: Minimum requirements to thermal insulation.

HONGOS ENDOFITOS EN PASTOS PDF

interaciones entre hongos endofitos de pastos y bacterias fijadoras de nitrógeno. In: Aportes de la microbiología a la producción de cultivos (Eds M. Díaz-Zorita. Relación entre cuerpos de lípidos y hongos endofitos de las inclusiones de lípidos en este tipo de plantas, las cuales se conservan en pastos y matorrales. Presencia de hongos endofitos (Neotyphodium spp.) en gramineas El endofito de raigras anual. Actas de la E1 pasto: El recurso mas barato. INTA EEA.

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Sistema OJS – Metabiblioteca. Publicado Jan 8, Hongos solubilizadores de fosfatos en fincas ganaderas del departamento de sucre.

Solubilization of Phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus TrichodermaharzianumRifai Santa fe de Bogota: Fungal Endophytes of Grasses: Editorial panamericana, p — A, p — Phosphate Stree in cultures and field populations of Dinoflagellateprorocentrum minimum detected by a single- cell alkaline phosphate assay.

Applied and Enviromental Microbiology.

Bioactivation of peorlysoluble phosphate rocks with a phosphorus — solubilizing fungus. Soil SciencieSociety of America Journal.

Fondo Fen Colombia, p 56 — Applied and Environmental Microbiology; Solubilization of insoluble inorganic phosphate by a soil — inhabiling fungus Formitopsis sp.

Phosphate — solubilizing bacteria and fungi various cultivate and virgin.

Relación entre cuerpos de lípidos y hongos endofitos

Revista Pesquisa Agropecuaria Brasileira. La ciencia del suelo y su manejo. McGraw — Hill, p — Avalaibility of Phosforus in a calcareous soil treated with rock-phosphat dissolving fungi. Characterization of a Highly Termostable Alkaline phsosphate from the Euryarchaceonpyrococcusabissi.

Applied and Enviromental microbiology, 67